Nucleic acids are first associated with magnetic nanoparticles. Gene Transfer: Method 4. Calcium Phosphate Co-Precipitation: This technique is used for the transfection of plant and mostly animal cells.
In this system, magnetic nanoparticles loaded with pure plasmid DNA carrying functional genes were delivered into pollens via pollen apertures in the presence of a magnetic field.
At the same time, a number of transformation methods based on pollen grains have been developed, such as electroporation, biolistic bombardment, ultra-sonication, and Agrobacterium incubation in plant pollens. Procedure: In this technique the recombinant DNA, which is negatively charged at a near neutral pH because of its phosphodiester backbone, is mixed with the lipid molecules with positively charged cationic head groups.
In this chapter, we have presented a protocol of pollen-based transformation technology in cotton, "pollen magnetofection," to directly produce transgenic seeds without tissue culturing. Top 13 Methods of Gene Transfer With Diagram Article shared by : The following points highlight the top thirteen methods of gene transfer.
Principle: The phospholipid molecules of the plasma membrane are not static. Afterward, these magnetofected pollens were used for pollination, to produce transformed seeds. The transfected cells are then selected by suitable methods.
The recombinant DNA can pass through these transient pores before they close. Sonoporation: Sonoporation, or cellular sonication, is the use of sound typically ultrasonic frequencies for the transfer of recombinant DNA into the target host cell. This results in the formation of recombinant DNA-calcium phosphate complex which appears as a thin precipitate.
Gene Ther — Dobson J Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery.